![]() ![]() ![]() UV Transilluminator Imaging of Fluorescently Stained Protein Gel The imager offers convenient touch screen interface and is compact, requiring the same bench space as a digital scanner, but also provides UV transilluminator capabilities.įigure 1. The image acquisition process is a motorized and computer-controlled operation except for the filter wheel which requires manual manipulation. ![]() Overall, Alpha Innotech’s Red Imaging System captures quality digital images of protein or DNA stained gels with a UV transilluminator and/or white light. The red Imaging System uses LED white lights mounted in the back of the instrument to illuminate the gels which results in a slight exposure gradient across the gel (Figure 2). The image is captured in about half the time required to adjust levels and scan images on a flatbed scanner. When imaging colorimetric stains (e.g., Coomassie and silver stains), the Red Imager-acquired images are of similar quality to a flatbed scanner. The Red Imaging System produces excellent images of protein gels stained with fluorescent UV excitable stains (Figure 1) and fluorescent DNA stains using the UV transilluminator. ![]() The imaging system gives you a numerical output for the exposure time but it would be beneficial to also have numerical outputs for the aperture, zoom and focus settings. The simple interface makes image acquisition quick and easy. The operator can select the illumination source (UV transilluminator or white light), aperture, zoom, focus and exposure time. The Red Imaging System captures quality images of stained gels with minimal adjustments. Images can be saved to a network through a LAN connection, a USB flash drive or printed directly by connecting a digital thermo printer. This cuts down on the required bench space and the need for yet another computer in the laboratory. The unit has built-in software and a user friendly touch screen interface allowing the Red Imager to be a stand alone unit that does not require a computer connection. The instrument takes up the same bench area as a flatbed scanner. Because a digital record of the gel is more convenient for obtaining publishable images, the need for versatile, low cost, personalized scanners and imagers has increased.Īlpha Innotech’s Red™ Imaging System is a quality UV transilluminator and white light imager for capturing images of stained DNA and protein gels. In the past, gels were either dried and kept as a record or photographed however, now imaging instruments such as scanners are more prevalent. Following electrophoresis, biomolecules are typically stained with fluorescent or colorimetric stains to visualize bands. When working with proteins and DNA, one of the first steps in any work flow or experiment is to separate the biomolecules by size on a SDS-PAGE or agarose gel. ![]()
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January 2023
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